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目的:探讨红霉素对哮喘大鼠NF-κB、Th2类细胞因子的影响。方法:分离、培养正常对照组、哮喘组、红霉素处理组大鼠的外周血单核细胞(PBMCs)。分别采用ELISA法检测细胞上清液中IL-4、IL-5、IL-13浓度。逆转录聚合酶链反应(RT-PCR)、Western blotting技术检测PBMCs NF-κB mRNA、NF-κB蛋白的表达。结果:哮喘组PBMCs mRNA、NF-κB蛋白表达水平及细胞上清液中IL-4、IL-5、IL-13的浓度较正常对照组显著升高(P<0.05),红霉素处理组NF-κB mRNA、NF-κB蛋白表达水平及细胞上清液中IL-4、IL-5、IL-13的浓度较哮喘组显著降低(P<0.05)。通过直线相关分析发现,PBMCs NF-κB蛋白与细胞上清液中IL-4、IL-5、IL-13的表达水平之间均呈显著正相关(r分别为0.78、0.86、0.71,P均<0.05)。结论:NF-κB可能参与支气管哮喘的病理生理过程。红霉素可能通过作用于NF-κB这一靶点发挥其免疫调节作用。
Objective: To investigate the effect of erythromycin on NF-κB and Th2 cytokines in asthmatic rats. Methods: Peripheral blood mononuclear cells (PBMCs) were isolated and cultured in normal control, asthma and erythromycin groups. The concentrations of IL-4, IL-5 and IL-13 in the supernatant were measured by ELISA. Reverse transcription polymerase chain reaction (RT-PCR) and Western blotting were used to detect the expression of NF-κB mRNA and NF-κB in PBMCs. Results: The levels of IL-4, IL-5 and IL-13 in PBMCs and NF-κB in asthma group were significantly higher than those in normal control group (P <0.05) The levels of NF-κB mRNA, NF-κB protein and IL-4, IL-5 and IL-13 in the cell supernatant were significantly lower than those in the asthma group (P <0.05). Linear correlation analysis showed that there was a significant positive correlation between the expression levels of IL-4, IL-5 and IL-13 in PBMCs NF-κB protein and cell supernatant (r = 0.78,0.86,0.71, P <0.05). Conclusion: NF-κB may be involved in the pathophysiological process of bronchial asthma. Erythromycin may exert its immunomodulatory effect by acting on the target of NF-κB.