论文部分内容阅读
用~(51)Gr释放、~3H-TdR掺入抑制及混合接种等方法观察了LACA纯系小鼠腹腔巨噬细胞体内体外对于S180肿瘤细胞的细胞毒效应以及胸腺肽对此作用的影响。结果表明,正常鼠和带瘤鼠的巨噬细胞在体外均可抑制或杀伤肿瘤细胞,E/T比例影响这一效应,E/T比例<5:1时巨噬细胞促进S180细胞的体外增殖。与单独应用环磷酰胺相比较,胸腺肽与环磷酰胺合用可增强巨噬细胞的细胞毒功能。正常鼠的巨噬细胞在体内混合接种实验中无作用,带瘤鼠的巨噬细胞在E/T比例为5:1时可延长生存期,提高存活率;而经胸腺肽处理的带瘤鼠的巨噬细胞在E/T比例为2.5:1时即可延长生存期。胸腺肽体外不能直接激活巨噬细胞,而Con A条件培液则具有这一功能。提示胸腺肽可通过一间接途径对带瘤鼠的巨噬细胞的抗瘤功能表现某种程度的调节作用。
The effects of ~(51)Gr release, ~3H-TdR incorporation inhibition and mixed inoculation on the cytotoxicity of LACA pure mouse peritoneal macrophages in vitro on S180 tumor cells and the effect of thymosin on this effect were observed. The results showed that macrophages from normal mice and tumor-bearing mice can inhibit or kill tumor cells in vitro. E/T ratio influences this effect. When E/T ratio<5:1, macrophages promote the proliferation of S180 cells in vitro. . The combination of thymosin with cyclophosphamide enhances the cytotoxicity of macrophages compared with cyclophosphamide alone. The macrophages of normal mice had no effect in mixed inoculation experiments in vivo, and macrophages with tumor mice prolonged the survival time and improved the survival rate at an E/T ratio of 5:1. The thymosin-treated mice with tumors Macrophages can prolong survival when E/T ratio is 2.5:1. Thymosin does not directly activate macrophages in vitro, whereas Con A conditional cultures have this function. It is suggested that thymosin can regulate the anti-tumor function of tumor-bearing mouse macrophages through an indirect pathway.