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目的 探讨树突状细胞 (DC)与慢性丙型肝炎患者体内病毒持续感染的关系。方法 从外周血分离单核细胞 ,用含粒细胞、巨噬细胞集落刺激因子 (GM CSF) ,白细胞介素 (IL) 4的AIM V无血清培养基诱导培养DC。观察DC外部形态和超微结构 ,检测DC表型 ,进行混合淋巴细胞反应(MLR) ,检测DC诱导同种异体静止T细胞增殖的能力和上清中细胞因子水平。结果 实验组DCCD86表达率为 (5 2 4± 13 3) % ,明显低于对照组的 (83 7± 15 8) % (P <0 0 1) ;实验组DC诱导同种异体静止T细胞增殖的能力每分钟液闪计数值 (cpm)为 192 4 5± 2 788,明显低于对照组的 2 6 5 2 9± 32 18(P <0 0 1) ;实验组MLR中IL 12p40 平均值为 (40 4± 10 3)ng/L ,干扰素γ平均值为 (7891± 82 1)ng/L ,分别低于对照组 (80 2± 2 0 5 )ng/L和 (13490± 15 5 4 )ng/L(P <0 0 0 5 )。结论 慢性丙型肝炎患者DC功能低下 ,提示与HCV持续感染有关。
Objective To investigate the relationship between dendritic cells (DC) and persistent virus infection in patients with chronic hepatitis C virus. Methods Mononuclear cells were isolated from peripheral blood and cultured in AIM V serum - free medium containing granulocyte, macrophage colony - stimulating factor (GM CSF) and interleukin (IL) 4. The morphology and ultrastructure of DCs were observed, the DC phenotypes were detected, mixed lymphocyte reaction (MLR) was performed, the ability of DC to induce allogeneic T cell proliferation and cytokine levels in supernatants were detected. Results The expression of DCCD86 in the experimental group was (52.4 ± 13.3)%, which was significantly lower than that in the control group (83 7 ± 15 8)% (P <0.01). DC induced the proliferation of allogeneic T cells (Cpm) was 192 45 ± 2 788, which was significantly lower than that of the control group (P <0.01). The average value of IL 12p40 in the MLR of the experimental group was (40 4 ± 10 3) ng / L and mean interferon γ (7891 ± 82 1) ng / L were significantly lower than those of the control group (80 2 ± 20 5 ng / L) and (13490 ± 15 5 4 ) ng / L (P <0 0 0 5). Conclusions DC in patients with chronic hepatitis C is poorly functional, suggesting that persistent infection with HCV.