推导了用荧光各向异性法测定环糊精包络物平衡结合常数的方程式,并用于测定芘-β-环糊精包络物的结合常数,获得了满意的结果,依据推得的方程式并结合各向异性～环糊精浓度曲线讨论了环糊精包络物存在形式及主-客体作用方式,预计了以蔡衍生物为配体的金属离子分析方法应用β-环糊精的可能性。 The equations for determining the equilibrium binding constant of the cyclodextrin inclusion complex by fluorescence anisotropy were derived and used to determine the binding constants of the pyrene-β-cyclodextrin envelope. Satisfactory results were obtained based on the derived equations Combined with the anisotropy ~ cyclodextrin concentration curves, the existence form of the cyclodextrin inclusion complex and the mode of action of the host-guest are discussed. The possibility of applying β-cyclodextrin to the metal ion analysis method using the Cai derivative as a ligand is forecasted .