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目的探讨Oddi括约肌中神经细胞的变化与重症急性胰腺炎(SAP)之间的关系。方法通过牛磺胆酸钠灌注的方式制备兔急性胰腺炎模型。采用免疫组化方法检测一氧化氮合成酶(NOS)和血管活性肠肽(VIP)在Oddi括约肌神经细胞中的表达。结果对照组肌间神经丛神经细胞的(45.83±2.17)%为NOS阳性细胞,(52.46±2.47)%为VIP阳性细胞,(22.73±1.95)%的神经细胞NOS和VIP均呈阳性表达。SAP组肌间神经丛神经细胞的(11.26±0.93)%为NOS阳性细胞,(28.62±2.83)%为VIP阳性细胞。SAP组的NOS和VIP阳性神经细胞的比例均明显低于对照组(P<0.01)。结论 NOS和VIP阳性神经细胞大量存在于Oddi括约肌;SAP时NOS及VIP阳性神经细胞比例均降低,可能是SAP时Oddi括约肌活动能力降低的原因。
Objective To investigate the relationship between changes of neurons in Oddi sphincter and severe acute pancreatitis (SAP). Methods Rabbit acute pancreatitis model was prepared by sodium taurocholate infusion. Immunohistochemistry was used to detect the expression of nitric oxide synthase (NOS) and vasoactive intestinal peptide (VIP) in the sphincter of Oddi. Results (45.83 ± 2.17)% of the myenteric plexus neurons in the control group were NOS positive cells, (52.46 ± 2.47)% were VIP positive cells, and (22.73 ± 1.95)% of the nerve cells were positive for NOS and VIP. (11.26 ± 0.93)% of myenteric plexus neurons in SAP group were NOS positive cells, (28.62 ± 2.83)% were VIP positive cells. The proportion of NOS and VIP positive neurons in SAP group were significantly lower than that in control group (P <0.01). Conclusions NOS and VIP positive neurons exist in Oddi sphincter in large numbers. The proportion of NOS and VIP positive neurons in SAP is decreased, which may be the reason of decreasing sphincter of Oddi in SAP.