由美味猕猴桃东山峰 79- 0 9品种诱导产生的 4种类型愈伤组织 ,其分离原生质体的效果不同 :A型愈伤组织分离的原生质体数量多 ,但活力较低 ;B型愈伤组织分离的原生质体不仅数量较多 ,而且活力也高 ;C型愈伤组织分离的原生质体数量少 ,但活力高 ;D型愈伤组织分离的原生质体数量较多 ,但活力低 The results showed that the four types of callus induced by the kiwifruit Dongshanfeng 79- 0 9 were different in their effect on protoplast isolation: type A callus had more protoplasts isolated but lower viability; type B callus The isolated protoplasts were not only abundant, but also highly viable. The number of protoplasts isolated from type C callus was small, but their viability was high. However, the number of protoplasts isolated from type D callus was higher but the viability was lower