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目的研究红景天苷对压力负荷型慢性心衰大鼠心电图的作用及可能的机制。方法 30只雄性SD大鼠随机分成假手术组(S组)、心衰组(HF组)、红景天苷组(SAL组),每组10只大鼠。HF组、SAL组采用腹主动脉缩窄法制备模型,S组只穿线不缩窄。SAL组术后12周给予红景天苷皮下注射,60 mg/kg·d,共30 d。假手术组和心衰组给予同等剂量的生理盐水。给药30 d后,Ⅱ导联心电图检测肢体导联并统计分析数据。将大鼠麻醉后,腹主动脉抽血离心后取血清,按试剂盒方法测定超氧化物歧化酶(SOD)、B型钠尿肽(BNP)、丙二醛(MDA)。结果术后12周,心电图检测显示几个特点:SAL组和HF组比较:1)心率和P波振幅有明显降低(P<0.05);2)R波振幅明显减小(P<0.05);3)心电图的J点位移明显降低(P<0.05),而ST高度则明显抬高(P<0.05)。血清酶的检测结果发现,和心衰组相比,SAL组大鼠血清中的SOD值明显增加(P<0.05),BNP值则明显降低(P<0.05)。结论红景天苷可以改善心衰大鼠的心功能,减轻心肌细胞的损伤,其机制可能与抗氧化作用相关。
Objective To study the effect of salidroside on ECG in chronic stress heart failure rats and its possible mechanism. Methods Thirty male Sprague-Dawley rats were randomly divided into sham operation group (S group), heart failure group (HF group) and salidroside group (SAL group), 10 rats in each group. HF group and SAL group were prepared by abdominal aorta stenosis model, S group only threading does not narrow. SAL group was given salidroside subcutaneously 12 weeks after operation, 60 mg / kg · d for 30 days. Sham operation group and heart failure group were given the same dose of saline. After administration for 30 days, lead Ⅱ ECG was used to detect limb lead and statistical analysis of the data. After the rats were anesthetized, blood was drawn from the abdominal aorta and centrifuged. The superoxide dismutase (SOD), B type natriuretic peptide (BNP) and malondialdehyde (MDA) were determined by kit method. Results After 12 weeks of operation, electrocardiogram revealed several characteristics: SAL group and HF group: 1) the amplitude of heart rate and P wave were significantly decreased (P <0.05); 2) the amplitude of R wave was significantly decreased (P <0.05); 3) The J point displacement of electrocardiogram decreased significantly (P <0.05), but the ST height was significantly increased (P <0.05). Serum enzyme test results showed that serum SOD increased significantly (P <0.05) and BNP decreased significantly (P <0.05) in SAL group compared with heart failure group. Conclusion Salidroside can improve cardiac function in heart failure rats, reduce myocardial cell injury, the mechanism may be related to antioxidant effects.