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探讨黄芩苷体外抗马立克氏病病毒(MDV)作用,应用细胞病变观察法(CPE)观察MDV对鸡胚成纤维细胞(CEF)的影响,采用Reed-Muench法计算MDV细胞半数感染量(TCID50),以CEF为宿主细胞,应用四甲基偶氮唑蓝(MTT)比色法从黄芩苷对MDV的增殖抑制、感染阻断以及直接灭活3个给药途径探讨黄芩苷抗MDV作用。结果显示:MDV对CEF的TCID50为10-4.5/0.1 m L;3种给药途径下,黄芩苷浓度在312.5~78.13μg/m L范围都具有较好的抗MDV感染作用,并且感染阻断途径下黄芩苷浓度为312.5~156.25μg/m L时能够促进细胞的生长,直接灭活途径下黄芩苷浓度为312.5μg/m L时能够促进细胞的生长。其中感染阻断与直接灭活途径给药比增值抑制途径给药的抗MDV感染作用效果要好,直接杀灭途径给药时的病毒抑制率最高。
To investigate the effect of baicalin against MDV in vitro, the effect of MDV on chicken embryo fibroblasts (CEF) was observed by cytopathic effect observation (CPE). The half number of infected MDV cells (TCID50) was calculated by Reed- To investigate the anti-MDV effect of baicalin by CEF as host cell, MTT colorimetry was used to study the inhibitory effect of baicalin on MDV proliferation, infection and direct inactivation. The results showed that the TCID50 of MDV to CEF was 10-4.5 / 0.1 m L; baicalin had a good anti-MDV infection effect in the range of 312.5-78.13 μg / mL under the three administration routes, and the infection was blocked Under the baicalin concentration of 312.5 ~ 156.25μg / m L, the growth of cells was promoted. When baicalin concentration was 312.5μg / m L, the growth of cells was promoted by direct inactivation pathway. Infection-blocking and direct inactivation routes were more effective than anti-MDV infection in the route of value-added inhibition, and the highest rate of virus suppression was achieved in the direct route of killing.