肝细胞移植和生物人工肝装置的核心问题是具有生物活性的足够数量肝细胞来源,肝细胞来源是解决肝衰竭患者的关键。1.成熟肝细胞:成熟肝细胞,特别是自体肝细胞,一经移植到肝脏等器官,就能马上发挥肝细胞功能。新鲜分离人肝细胞是影响临床效果的重要因素,由于没有充足的肝脏用来提取新鲜肝细胞,肝细胞培养增殖技术也就尤为重要。为了达到 The core issue of hepatocyte transplantation and bioartificial liver devices is the availability of a sufficient number of biologically active sources of hepatocytes, the source of which is the key to resolving the problem of patients with liver failure. 1. Mature liver cells: mature liver cells, especially autologous hepatocytes, once transplanted to the liver and other organs, can immediately play a hepatocyte function. Fresh isolation of human hepatocytes is an important factor affecting the clinical efficacy. Because there is not enough liver to extract fresh hepatocytes, the technology of hepatocyte proliferation is also very important. In order to achieve