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目的 研究肺癌 P53基因 DNA水平和蛋白水平的突变。方法 应用 PCR- SSCP银染技术检测了 5 4例肺癌标本。结果 2 2例 DNA水平突变阳性 ,突变率为 41%。 32例在核蛋白水平突变 ,突变率 5 9%。其中 2 2例 DNA突变阳性者 ,核蛋白突变均阳性。而 32例 DNA水平突变阴性者 ,核蛋白突变阳性者 9例 ,占 2 8%。P53 DNA突变与核蛋白的突变之间有显著相关性 ,并且两者都与患者的淋巴结转移有关 (P<0 .0 1)。结论 PCR- SSCP是一种高灵敏快速检测 P53是否存在异常的有效方法。但是 ,P53突变阳性患者预后较差。
Objective To study the mutation of DNA and protein levels of P53 gene in lung cancer. Methods PCR-SSCP silver staining was used to detect 54 lung cancer specimens. Results DNA level mutations were positive in 21 cases with a mutation rate of 41%. 32 cases of mutations in the nuclear protein level, the mutation rate of 59%. Among them, 22 cases of DNA mutation were positive, and the mutation of nucleoprotein was positive. In 32 cases with negative DNA level mutations, 9 cases were positive for nucleoprotein mutations, accounting for 28%. There was a significant correlation between P53 DNA mutations and mutations in the nucleoprotein, and both were associated with lymph node metastases (P <0.01). Conclusion PCR-SSCP is a sensitive and rapid detection of P53 is an effective method. However, patients with positive P53 mutations have a poor prognosis.