若按卫生部编著的《药品卫生标准检验方法》一书中的操作,在测定杂菌总数时,倾注入平皿的培养基温度为45—50℃,但在实际操作中,往往凭手感来掌握,因此温度差异较大,特别是在较多样品同时进行测定时,前后的温度相距尤为明显。为了观察其带来的误差,我们作了如下实验。 According to the book edited by the Ministry of Health, “the method of testing the health standards,” a book in the operation, in determining the total number of bacteria, poured into the plate of the medium temperature is 45-50 ℃, but in practice, often by hand to grasp , So the temperature difference is large, especially when more samples are measured at the same time, before and after the temperature is particularly obvious. In order to observe the error caused by it, we made the following experiment.