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目的 研究异丙酚对猕猴失血性休克与复苏过程中所致再灌注肺损伤的保护作用。方法 将 12只猕猴随机分为对照组 (n =6 )和异丙酚组 (n =6 )。异丙酚组在放血前 10min用计算机靶控输注技术使猕猴血浆异丙酚浓度达到 8mg/L并维持此水平。对两组动物用相同方法制作失血性休克模型 ,维持休克 2h后复苏 ,回输全部自体抗凝血及 2倍放血量的生理盐水 ,复苏后 2h处死动物取肺组织进行干湿重测定及病理检查。分别于放血前、休克 2h和复苏 2h测定血流动力学参数 ,并取静脉血标本测定丙二醛 (MDA)、超氧化物歧化酶 (SOD)和乳酸 (LA)含量。结果 对照组猕猴在休克 2h和复苏 2h时的平均肺动脉压 (MPA)、肺血管阻力指数 (PVRI)均明显高于异丙酚组 (P <0 0 5 ) ,异丙酚组猕猴的MDA、LA总体水平均明显低于对照组 (P <0 0 5 ) ,对照组动物肺组织湿重 /干重比值明显大于异丙酚组 (P <0 0 5 ) ,病理观察发现异丙酚组肺组织水肿较对照组轻。结论 异丙酚对猕猴缺血再灌注所引起的肺组织损伤有一定保护作用。
Objective To investigate the protective effect of propofol on lung injury induced by hemorrhagic shock and resuscitation in rhesus monkeys. Methods Twelve cynomolgus monkeys were randomly divided into control group (n = 6) and propofol group (n = 6). In the propofol group, plasma propofol concentration of 8 mg / L was maintained and maintained at 10 min before bloodletting by computer-controlled infusion. The hemorrhagic shock model was made by the same method in both groups, and the rats were resuscitated 2h after shock and all autologous anticoagulant blood and 2 times blood volume were returned to normal saline. The animals were sacrificed at 2 hours after resuscitation for lung wet and dry weight determination Pathological examination. The hemodynamic parameters were measured before blood exsanguination, shock 2h and resuscitation 2h, and the contents of malondialdehyde (MDA), superoxide dismutase (SOD) and lactate (LA) were determined by venous blood samples. Results The average pulmonary arterial pressure (MPA) and pulmonary vascular resistance index (PVRI) in control group were significantly higher than those in propofol group (P <0.05) at 2 h and 2 h after resuscitation. The contents of MDA, (P <0.05). The lung wet weight / dry weight ratio in the control group was significantly higher than that in the propofol group (P <0 05). The pathological findings showed that the propofol group lung Tissue edema lighter than the control group. Conclusion Propofol can protect the lung tissue from ischemia-reperfusion injury induced by cynomolgus monkeys.