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对甲胺磷敏感性的田间监测结果显示,绒茧蜂存在着抗性演化,毒力生物测定结果与AChE的K_i值的监测结果呈明显的相关性,每年9月至次年2月期间AChE敏感性最低,8月期间敏感性最高。甲胺磷可显著地抑制绒茧蜂AChE、CarE和GSTs的活性。PB和TPP对AChE的活体抑制率极低,但PB可强烈抑制CarE的活性,而TPP仅在高浓度时对CarE有较显著的抑制作用,PB对甲胺磷有显著的增效作用,而TPP对甲胺磷无增效作用。AChE的K_m、V_(max)及K_i值研究结果表明,田间绒茧蜂对有机磷和氨基甲酸酯的抗性与AChE对杀虫剂的不敏感性有关。由此认为,绒茧蜂对有机磷的抗性主要与其最重要的靶标酶AChE的敏感性改变及多功能氧化酶有关。
Methamidophos sensitivity of the field monitoring results show that there is resistance evolution callus, virulence bioassay results and AChE K_i value of the monitoring results showed a significant correlation between September to February next year AChE Lowest sensitivity, most sensitive during August. Methamidophos significantly inhibited the activity of AChE, CarE and GSTs in Culex. PB and TPP had a very low in vivo inhibition rate of AChE, but PB strongly inhibited the activity of CarE, while TPP only had a significant inhibitory effect on CarE only at high concentration, while PB had a significant synergistic effect on methamidophos, TPP no synergistic effect on methamidophos. The results of K_m, V_ (max) and K_i values of AChE indicated that the resistance of P. americana to organophosphorus and carbamate was insensitive to insecticides. Therefore, the resistance of C. calyciflor to organophosphorus was mainly related to the change of sensitivity of its most important target enzyme AChE and multifunctional oxidase.