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建立了免疫印渍法检测人白细胞中c-myc原癌基因蛋白方法,利用生物素-链菌素亲生物素蛋白系统提高检测敏感性;上样蛋白量约合细胞数为3×106~4×106。采用光密度扫描进行癌蛋白的半定量分析,并已用于白血病细胞的检测
A method for the detection of c-myc proto-oncogene protein in human leukocytes by immunoblotting was established, and the detection sensitivity was improved by the biotin-streptavidin protein system; the amount of loaded protein was approximately 3×10 6 to 4 ×106. Semi-quantitative analysis of oncoproteins using densitometry and detection of leukemia cells