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慢性中耳炎常为分泌性中耳炎所后贻,但其活动期显然与感染有关。为了解慢性中耳炎和分泌性中耳炎的中耳粘膜内炎性细胞的浸润情况有无异同,作者于乳突根治术时取12例乳突和中耳粘膜,对其固有层进行了观察。取下的标本浸入消毒的生理盐水后再按Ranki等(1980)介绍的方法——以组织化学的ANAE(α-萘醋酸酯酶)标记法进行处理,以便观察免疫活性细胞反应。经上述方法处理后,T淋巴细胞及单核吞噬细胞呈ANAE阳性,B淋巴细胞则多为ANAE阴性。此外还观察了粒性白细胞及浆细胞的存在情况。结果:病变轻,炎症不明显者(2例),粘
Chronic otitis media often secretory otitis media after Yi, but its activity is clearly related to infection. In order to understand whether inflammatory cells in the middle ear mucosa of patients with chronic otitis media and secretory otitis media had similar infiltration, the authors observed 12 cases of papilla and middle ear mucosa during radical mastectomy. The removed specimen was immersed in sterilized saline and then treated according to the method described by Ranki et al. (1980) - histochemical ANAE (? -naphthaleneacetate esterase) labeling method in order to observe immunocompetent cellular responses. After the above method, T lymphocytes and mononuclear phagocytes were ANAE positive, B lymphocytes are mostly ANAE negative. In addition, we observed the presence of granulocytes and plasma cells. Results: mild lesions, inflammation was not obvious (2 cases), sticky