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目的探讨人胃癌组织中Tie2mRNA和Tie2蛋白的表达及其与血管生成的关系。方法以42例胃癌患者癌组织和37例癌旁组织和30例正常胃粘膜组织为研究对象,用RT-PCR技术和免疫组化S-P法检测Tie2mRNA、Tie2蛋白和CD34的表达情况。结果胃癌组织和癌旁组织中Tie2 mRNA和Tie2蛋白的表达均明显高于正常胃组织,比较差异均有统计学意义(P<0.05)。Tie2蛋白随癌肿瘤分化程度降低和淋巴结转移明显增高(P<0.05),但与性别和年龄无明显相关(P>0.05)。Tie2蛋白阳性表达的MVD显著高于Tie2蛋白阴性者,差异有统计学意义(P<0.01)。结论 Tie2在人胃癌组织中高表达,可能与肿瘤分化程度与淋巴转移有关,并与肿瘤血管生成关系密切。
Objective To investigate the expression of Tie2 mRNA and Tie2 protein in human gastric carcinoma and its relationship with angiogenesis. Methods 42 cases of gastric cancer tissue and 37 cases of paracancerous tissues and 30 cases of normal gastric mucosa tissues were studied. The expression of Tie2 mRNA, Tie2 protein and CD34 were detected by RT-PCR and immunohistochemical S-P method. Results The expressions of Tie2 mRNA and Tie2 protein in gastric cancer tissues and adjacent tissues were significantly higher than those in normal gastric tissues (P <0.05). Tie2 protein decreased with tumor differentiation and lymph node metastasis (P <0.05), but not with sex and age (P> 0.05). The MVD of Tie2 protein was significantly higher than that of Tie2 protein (P <0.01). Conclusion Tie2 is highly expressed in human gastric cancer tissues, which may be related to the degree of tumor differentiation and lymphatic metastasis and is closely related to tumor angiogenesis.