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目的探讨p27蛋白和增殖细胞核抗原(PCNA)在溃疡性结肠炎、结肠癌肠黏膜中的表达及其与细胞凋亡的关系。方法应用免疫组化SABC方法检测结肠癌、溃疡性结肠炎患者及正常人结肠肠黏膜中p27蛋白和PCNA的表达,用DNA原位末端标记TUNEL法检测标本中的细胞凋亡。结果结肠癌及溃疡性结肠炎组织中的PCNA表达水平明显高于正常结肠(P<0.05);p27蛋白阳性表达的结肠癌细胞平均凋亡指数(0.93±0.32)%高于p27蛋白阴性表达的细胞凋亡指数(0.39±0.18)%,差异有统计学意义(P<0.05),p27与结肠癌PCNA的表达无关。结论溃疡性结肠炎结肠黏膜标本PCNA表达呈强阳性且组织分布弥散提示结肠黏膜癌或有癌变倾向;p27蛋白表达可能不抑制结肠癌细胞的增殖,但有促使结肠癌细胞凋亡的趋势。
Objective To investigate the expression of p27 protein and proliferating cell nuclear antigen (PCNA) in ulcerative colitis and intestinal mucosa of colon cancer and its relationship with apoptosis. Methods Immunohistochemical SABC method was used to detect the expression of p27 protein and PCNA in colon and ulcerative colitis patients and normal colonic mucosa. The apoptosis of the specimens was detected by TUNEL method. Results The expression of PCNA in colon cancer and ulcerative colitis was significantly higher than that in normal colon (P <0.05). The average apoptosis index of p27 positive cells (0.93 ± 0.32)% was higher than that of p27 negative The apoptosis index (0.39 ± 0.18)%, the difference was statistically significant (P <0.05), p27 and PCNA colon cancer has nothing to do. Conclusions The expression of PCNA in colonic mucosa of ulcerative colitis is strongly positive and the distribution of tissue is diffusely suggestive of colorectal cancer. The expression of p27 protein may not inhibit the proliferation of colorectal cancer cells, but may promote the apoptosis of colon cancer cells.