目的　探讨p16基因与膀胱肿瘤细胞凋亡的关系。方法　应用SABC免疫组织化学法检测了 5 1例膀胱移行细胞癌p16基因的表达 ,同时用流式细胞仪检测其细胞凋亡情况。结果　p16阳性表达率为 47 1% (2 4/ 5 1) ,平均凋亡肿瘤细胞百分比为 1 5 % ,p16阳性表达的肿瘤组织凋亡细胞百分比 >1 5 %的发生率为 6 2 5 % ,p16阴性表达的肿瘤组织凋亡细胞百分比 >1 5 %的发生率为2 5 9% ,两者比较有显著性差异 (P <0 0 1)。结论　p16基因的表达与膀胱肿瘤细胞凋亡有关 ,p16基因可能参与膀胱肿瘤细胞凋亡的正向调节作用 Objective To investigate the relationship between p16 gene and apoptosis of bladder tumor cells. Methods SABC immunohistochemistry was used to detect the expression of p16 gene in 51 cases of transitional cell carcinoma of the bladder, and its apoptosis was detected by flow cytometry. Results The positive rate of p16 was 47 1% (2 4/5 1), the average percentage of apoptotic tumor cells was 15%. The percentage of apoptotic cells with p16 positive expression> 15% was 62.5% The percentage of apoptotic cells> 15% in p16 negative expression was 25.9%, which showed significant difference (P <0.01). Conclusion The expression of p16 gene is related to the apoptosis of bladder tumor cells. The p16 gene may be involved in the positive regulation of apoptosis of bladder tumor cells