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目的 :探查大鼠四氯化碳肝硬化形成早期红细胞变形指数、全血粘度等多项血液流变学指数的变化。方法 :将Wistar大鼠随机分组 ,肝组织HE和天狼猩红染色 ,下腔静脉取血检测红细胞变形指数、全血粘度等血液流变学参数。结果 :肝硬化 7w鼠的肝组织中有大量胶原纤维增生并开始形成假小叶。对照组和肝硬化组间红细胞数和全血粘度的差异不明显 ;实验 4～ 5w后 ,肝硬化组的红细胞变形指数、血浆纤维蛋白原和红细胞血红蛋白含量明显较对照组降低。结论 :四氯化碳大鼠肝硬化模型的红细胞变形指数等多项血液流变学参数在第 5w后出现明显异常 ,比形态学上出现典型肝硬化表现约提前了 2～ 3w时间 ,为临床肝硬化的早期诊断治疗提供了实验依据。
OBJECTIVE: To explore the changes of many indexes of hemorheology such as early erythrocyte deformability index, whole blood viscosity and so on in the formation of cirrhotic carbon tetrachloride (CCl4) in rats. Methods: Wistar rats were randomly divided into three groups: liver tissue HE and Sirius red staining; blood samples of inferior vena cava were used to detect hemorrheological parameters such as erythrocyte deformability index and whole blood viscosity. Results: A large number of collagen fibers proliferated in the hepatic tissue of cirrhotic mice and began to form pseudolobules. There was no significant difference between red blood cell count and whole blood viscosity in control group and cirrhosis group. After 4 ~ 5 w of experiment, the indexes of erythrocyte deformability, plasma fibrinogen and red blood cell hemoglobin in cirrhosis group were significantly lower than those in control group. CONCLUSIONS: A number of hemorheological parameters such as erythrocyte deformability index of cirrhotic rat model of CCl 4 appear obvious abnormalities after 5 weeks, which is about 2 ~ 3w earlier than morphological typical cirrhosis performance, and are clinically Early diagnosis and treatment of cirrhosis provides experimental evidence.