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从GenBank公共数据库中下载黄麻表达序列标签838条,利用SSRPrimer软件对其进行SSR位点查找,利用Primer 3.0软件设计66对SSR引物,通过琼脂糖凝胶研究这些SSR引物的PCR扩增特点,以检测其多态性。结果表明,66对SSR引物在黄麻属6个不同类型材料的扩增中,42(63.6%)对引物至少在2个材料之间存在多态性。(AT)n重复基元和(GC-)n丰富的三核苷酸重复基元多态性较高,可作为黄麻SSR标记引物设计的首选。黄麻EST-SSR标记开发效率较高,不仅可以丰富黄麻分子标记的数量,而且为剖析黄麻重要性状的遗传机制奠定基础,这对于黄麻的遗传基础研究具有重要应用价值。
838 ESTs were downloaded from the GenBank public database and SSR loci were searched using SSRPrimer software. 66 pairs of SSR primers were designed by Primer 3.0 software, and the PCR amplification characteristics of these SSR primers were studied by Test its polymorphism. The results showed that of 66 pairs of SSR primers, 42 (63.6%) were polymorphic to at least two of the primers in the amplification of six different genotypes of Juglans. (AT) n repeat motifs and (GC-) n trinucleotide repeat motifs rich in polymorphism, which can be used as the jute SSR primer primer design of choice. Jute EST-SSR marker development efficiency is high, not only can enrich the number of jute molecular markers, but also lay the foundation for the analysis of genetic mechanism of important traits of jute, which has important application value for the genetic basis of jute.