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目的联合应用止颤颗粒和美多芭,观察止颤颗粒是否具有增强美多芭治疗帕金森病(PD)的作用。方法采用经典的1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)诱发小鼠亚急性PD模型,105只小鼠,随机分为7组,每组15只,分别为对照组、模型组、止颤颗粒低剂量(3.5 g/kg)+美多芭(50 mg/kg)组、止颤颗粒中剂量(9.0 g/kg)+美多芭(50 mg/kg)组、止颤颗粒高剂量(22 g/kg)+美多芭(50 mg/kg)组、止颤颗粒(22 g/kg)组、美多芭(50 mg/kg)组。采用转棍法和爬杆法检测各组小鼠的行为学指标,采用HPLC法测定纹状体中多巴胺(DA)水平、免疫组化法检测黑质多巴胺神经元数量。结果止颤颗粒低剂量+美多芭组与美多芭组相比,纹状体内DA的水平、TH阳性神经元数目均明显提高,而且PD模型小鼠行为学障碍改善作用较明显。与模型组相比,止颤颗粒组小鼠的行为学障碍改善作用较明显。结论止颤颗粒可增强美多芭的疗效,具有一定的神经保护作用,止颤颗粒低剂量与美多芭合用可适当减少美多芭的用量。
Objective To observe the effects of Zhidan Granules on the treatment of Parkinson’s disease (PD) by using combination of defibrillators and metoprolol. Methods Subacute PD model mice were induced by classic 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). 105 mice were randomly divided into 7 groups of 15 The results were as follows: the control group, the model group, Zhixuan Granules at low dose (3.5 g / kg) + metoprolol (50 mg / kg) (22 mg / kg), high dose of defibrillators (22 g / kg) + metoprolol (50 mg / kg), defibrotide (22 g / . The behavioral indexes of mice in each group were detected by the rotary stick method and climbing pole method. The level of dopamine (DA) in the striatum was determined by HPLC and the number of dopamine neurons in substantia nigra was detected by immunohistochemistry. Results Compared with the model group, the level of DA and the number of TH-positive neurons were significantly increased in the low-dose Zhidan Granules + memantine group compared with the midodal group, and the behavioral dysfunction improved significantly in the PD model mice. Compared with the model group, Zhidan particles group mice behavioral disorders to improve the role of more obvious. Conclusion Zhidan Granules can enhance the efficacy of the United States and the United States Toba, with some neuroprotective effects, low doses of defibrillators and metoprolol may be appropriate to reduce the amount of the United States and Toba.