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以秋水仙素为诱变剂,此较了不同浓度和时间对苎麻不同器官或部位进行处理的诱导效果。种子处理,由于胚根生长受到严重抑制,生活力差,成活率低,有效诱导率仅0.65％。首次成功地进行了苎麻实生幼苗茎尖处理,平均诱导率达9.04％,为国内已报道最高诱导率的5倍。处理的直接效应多数形成嵌合体,采用嫩梢扦插等繁殖方法即可分离完全多倍体。根据苎麻多倍体主要特征特性,采取大田目测或结合成熟花粉粒镜检进行初选,最后以染色体计数为准的鉴定程序,既省时又可靠。
Colchicine as a mutagen, which compared different concentrations and time of ramie in different organs or parts of the induction effect. Seed treatment, due to severe inhibition of radicle growth, poor life, low survival rate, the effective induction rate of only 0.65%. For the first time, the shoot tips of ramie seedlings were successfully treated with the average induction rate of 9.04%, which is 5 times of the highest induction rate reported in China. Most of the direct effects of treatment formed chimeras, the use of tender shoots and other reproductive methods can be completely polyploid separation. According to the main characteristics of ramie polyploid, polo ralph lauren pas cher, field observations or combined with mature pollen microscopic examination for primaries, and finally to the chromosome count-based identification procedures, both time-saving and reliable.