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目的研究、探讨血小板源性生长因子（ｐｌａｔｅｌｅｔｄｅｒｉｖｅｄｇｒｏｗｔｈｆａｃｔｏｒ，ＰＤＧＦ）和肝素对人主动脉平滑肌细胞（ｈｕｍａｎａｏｒｔａｓｍｏｏｔｈｍｕｓｃｌｅｃｅｌ，ｈＡＳＭＣ）增殖、胶原蛋白的合成、分泌以及Ⅰ、Ⅲ型前胶原ｍＲＮＡ表达和转移生长因子－β（ｔｒａｎｓｆｏｒｍｉｎｇｇｒｏｗｔｈｆａｃｔｏｒ－β，ＴＧＦ－β）ｍＲＮＡ表达的调节作用。方法３Ｈ－ＴｄＲ，３Ｈ－脯氨酸掺入及Ｎｏｒｔｈｅｒｎｂｌｏｔ分析。３Ｈ－ＴｄＲ，３Ｈ－脯氨酸掺入的比较采用ｔ检验。结果ＰＤＧＦ能促进ｈＡＳＭＣＤＮＡ的合成和胶原蛋白的合成、分泌；能上调Ⅰ、Ⅲ型前胶原ｍＲＮＡ表达和ＴＧＦ－βｍＲＮＡ表达。肝素能抑制ｈＡＳＭＣＤＮＡ合成和胶原蛋白的合成、分泌，还部分拮抗ＰＤＧＦ促进ｈＡＳＭＣＤＮＡ合成和胶原蛋白合成、分泌的作用，以及拮抗ＰＤＧＦ上调Ⅰ、Ⅲ型前胶原ｍＲＮＡ表达和ＴＧＦ－βｍＲＮＡ表达的作用。结论ＰＤＧＦ能通过上调Ⅰ、Ⅲ型前胶原ｍＲＮＡ表达和ＴＧＦ－βｍＲＮＡ表达促进平滑肌细胞（ｓｍｏｏｔｈｍｕｓｃｌｅｃｅｌ，ＳＭＣ）胶原蛋白的合成、分泌；肝素能抑制ＳＭＣＤＮＡ和胶原蛋白合成，还能拮抗ＰＤＧＦ的促ＳＭＣ胶原蛋白合成?
Objective To investigate the effects of platelet derived growth factor (PDGF) and heparin on proliferation, collagen synthesis and secretion of human aorta smooth muscle cells (hASMC) and the expression of type Ⅰ and Ⅲ procollagen mRNA and the expression of transforming growth factor-β (Transforming growth factor-β, TGF-β) mRNA expression regulation. Methods 3H-TdR, 3H-proline incorporation and Northern blot analysis. 3H-TdR, 3H-proline incorporation using t test. Results PDGF could promote the synthesis of hASMCDNA and the synthesis and secretion of collagen, and upregulate the expressions of procollagen type Ⅰ and Ⅲ mRNA and TGF-βmRNA. Heparin can inhibit hASMCDNA synthesis and collagen synthesis, secretion, but also partially antagonize PDGF promote hASMCDNA synthesis and collagen synthesis and secretion, as well as antagonize PDGF upregulated type Ⅰ, Ⅲ procollagen mRNA expression and TGF-βmRNA expression. Conclusion PDGF can promote the synthesis and secretion of collagen in smooth muscle cells (SMCs) by upregulating the expression of procollagen type Ⅰ and Ⅲ mRNA and TGF-β mRNA. Heparin can inhibit the synthesis of SMCDNA and collagen, and also antagonize the promotion of PDGF-stimulated SMC collagen Protein synthesis?