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目的:探讨新疆曼陀罗子(生品,炮制品)急性毒性及其生物碱(阿托品、东莨菪碱)含量间的关系。方法:(1)维吾尔医药学炮制法,制备新疆曼陀罗子(炮制品)。(2)选用小鼠,分别对新疆曼陀罗子(生品、炮制品)进行急性毒性试验;同时,高效液相色谱法,检测新疆曼陀罗子(生品、炮制品)阿托品、东莨菪碱含量。结果:(1)新疆曼陀罗子(生品、炮制品)对小鼠最大耐受剂量(MTD)分别为40g(生药)/kg、32g(生药)/kg。(2)新疆曼陀罗子(生品、炮制品)阿托品及东莨菪碱含量分别0.8392mg/g、0.2295mg/g,0.6374mg/g、0.1683mg/g。结论:初步认为,新疆曼陀罗子(生品)具有毒性,但不是剧毒;新疆曼陀罗子(生品)通过传统的维吾尔医药学炮制方法处理能降低其毒性,这可能与降低其阿托品及东莨菪碱含量有关。
Objective: To investigate the relationship between the acute toxicity of Datura manchurianus (raw and processed products) and the contents of alkaloids (atropine and scopolamine) in Xinjiang. Methods: (1) Uyghur medicine processing method, the preparation of Xinjiang Mandala (processed products). (2) The mice were used to conduct acute toxicity test on the Datura sericata (raw product, processed product) of Xinjiang. At the same time, high performance liquid chromatography (HPLC) was used to detect atropine, scopolamine content. The results showed that: (1) The maximum tolerated dose (MTD) of M. mandi in Xinjiang was 40g (crude drug) / kg and 32g (crude drug) / kg, respectively. (2) The contents of atropine and scopolamine in Mandala of Xinjiang were 0.8392mg / g, 0.2295mg / g, 0.6374mg / g and 0.1683mg / g, respectively. CONCLUSIONS: It is preliminarily believed that the Salamander (Salamanders) of Xinjiang is toxic, but not virulent. The treatment of Xinjiang Mandarins (crude) by traditional Uyghur medicine can reduce its toxicity, which may be related to the reduction of its toxicity Atropine and scopolamine content.