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目的制备包裹小分子干扰RNA(siRNA)-紫杉醇(PTX)的固体脂质纳米粒(SLNs)(siRNA-PTX-SLNs),考察其理化性质和包封率。方法采用薄膜超声法制备siRNA-PTX-SLNs;凝胶柱层析法分离SLNs、游离的PTX及游离的siRNA;HPLC法检测PTX的含量;荧光标记法检测siRNA的含量;测定粒径、电位,并在电镜下观察形状。结果 siRNA-PTX-SLNs中紫杉醇的包封率>96%;siRNA的包封率为56.65%±0.5%;SLNs的粒径、电位分别为155±0.23 nm、40±2.34 mV。结论成功制备了能同时有效包裹siRNA和PTX的siRNA-PTX-SLNs。
OBJECTIVE: To prepare solid lipid nanoparticles (SLNs) (siRNA-PTX-SLNs) encapsulating small interfering RNA (siRNA) -polysaccharide (PTX) and study their physico-chemical properties and entrapment efficiency. Methods Thin-film ultrasound was used to prepare siRNA-PTX-SLNs. The SLNs, free PTX and free siRNA were separated by gel column chromatography. The content of PTX was detected by HPLC. The content of siRNA was detected by fluorescent labeling. And observe the shape under electron microscope. Results The encapsulation efficiency of paclitaxel in siRNA-PTX-SLNs was> 96%. The entrapment efficiency of siRNA was 56.65% ± 0.5%. The diameters and potentials of SLNs were 155 ± 0.23 nm and 40 ± 2.34 mV, respectively. Conclusion siRNA-PTX-SLNs that can effectively encapsulate both siRNA and PTX were successfully prepared.