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【目的】探讨中国野生葡萄果皮和叶片中白藜芦醇(Resveratrol,Res)含量的特点,为进一步开发利用中国野生葡萄资源提供理论依据。【方法】以51个野生葡萄株系为材料,利用高效液相色谱法(HPLC)连续2 a测定成熟果皮和叶片中白藜芦醇的含量,并结合气候条件进行对比分析。【结果】在51个野生葡萄株系中,约60%的葡萄果皮中白藜芦醇含量高于叶片,株系间果皮、叶片Res含量(ω)存在很大差异,其中果皮中含量最高的‘双溪腺枝葡萄03’(2013年与2014年分别为67.82μg·g~(-1)和68.44μg·g~(-1))比含量最低的‘洪江刺葡萄04’(2013年0.08μg·g~(-1),2014年0.09μg·g~(-1))高855.5倍,叶片中含量最高的‘高山二号’(2013与2014年分别为10.27μg·g~(-1)和11.69μg·g~(-1))比含量最低的‘冯举沟桑叶葡萄02’高256.8倍。结合采样地2 a的气候条件分析,果皮中的Res含量几乎未受气候条件影响,而叶片中Res含量在采样的2 a间变化幅度较大。【结论】腺枝葡萄中的‘双溪腺枝葡萄03’‘芷江水腺枝葡萄’‘双溪腺枝葡萄01’及山葡萄中的‘山葡萄N43-3’‘长白9号’‘双优’等果皮富含白藜芦醇。研究为有效利用和开发野生葡萄资源中的白藜芦醇及促进功能性食品开发提供理论依据。
【Objective】 The purpose of this study was to investigate the characteristics of resveratrol (Res) content in the peel and leaves of wild grapes in China and to provide a theoretical basis for further exploitation and utilization of wild grapes in China. 【Method】 51 wild grapevine strains were used as materials. The content of resveratrol in mature peel and leaf was determined by high performance liquid chromatography (HPLC) for 2 consecutive days, and compared with the climatic conditions. 【Result】 Resveratrol content in peel of about 60% of the 51 wild grapevines was higher than that of leaves. There was a great difference in the content of Res (ω) in the peel and leaf between the strains, of which the content was the highest in the peel ’Hongjiang Thorn Grape 04’ with the lowest content of ’Shuangyi Ganzhi Grape 03’ (67.82μg · g -1 and 68.44μg · g -1 in 2013 and 2014 respectively) (0.08 in 2013 The results showed that ’Takayama No.2’ with the highest content of leaves in the leaves was 10.27 μg · g -1 (μg · g -1) in 2014 and 0.09 μg · g -1 in 2014, ) And 11.69 μg · g -1) were 256.8 times higher than that of ’Fengjuogou mulberry grape 02’ which was the lowest content. According to the analysis of climatic conditions during the sampling period of 2 years, the content of Res in the peel was almost unaffected by climatic conditions, while the content of Res in leaves varied greatly during the sampling period of 2 years. 【Conclusion】 The results showed that ’Shuangxi Ganzhi grapes 03’, Zhigan Shui gianzhi grapes ’, Shuangxi Ganzhi grapes 01’ and ’Shanbei N43-3’, Changbai 9 ’Shuangyou’ Such as peel is rich in resveratrol. The research provides a theoretical basis for the effective utilization and development of resveratrol in wild grape resources and the promotion of functional food development.