穿梭质粒P~(z189)转化原核细胞后,用甲基磺酸乙酯对转化的原核细胞进行诱变处理,使P~(z189)发生突变,突变的靶基因导致了再转化克隆在选择培养基上颜色改变。用温度梯度凝胶电泳技术检测突变子上的靶基因发现,当选择的试验条件合适时,这项技术能够检测出结构变异的DNA片段。 After the shuttle plasmid P~(z189) was transformed into prokaryotic cells, the transformed prokaryotic cells were subjected to mutagenesis with ethyl methanesulfonate to mutate P~(z189), and the mutated target gene led to retransformation and cloning in selective culture. Based on the color change. Detection of target genes on mutants using temperature gradient gel electrophoresis revealed that this technique can detect structurally altered DNA fragments when the selected test conditions are appropriate.