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以６０Ｃｏ－γ射线照射烟草优质感病品种的花药作为诱变手段，用烟草黑胫病疫霉菌粗毒素为筛选剂，对烟草花粉植株叶片的愈伤组织，进行抗病突变体细胞的筛选．试验表明：毒素对烟草愈伤组织的存活和增殖有明显的抑制作用，抑制程度随毒素浓度提高而加强，同一浓度对不同品种也存在着差异．综合毒素浓度对烟草愈伤组织抑制、增殖程度及筛选的有效性，初步认为：筛选红大和Ｋ３２６抗黑胫病突变体细胞的最适粗毒素浓度分别为３０％和４５％．所筛选出的抗病突变体细胞，其细胞水平的抗性与再生植株的抗病性鉴定相吻合．
The anther of 60Co-γ-rays was used to mutagenize the tobacco varieties with high quality susceptible varieties, and the callus of Tobacco pollen plant leaves was screened for disease-resistant mutants by screening the tobacco black shank Phytophthora capsici crude toxin. The results showed that: the toxin had a significant inhibitory effect on the survival and proliferation of tobacco callus, the degree of inhibition enhanced with the increase of the concentration of toxin, and the same concentration had different effects on different varieties. The results showed that the optimum concentrations of crude toxins of Rhizoctonia solani and K326 mutant were 30% and 45%, respectively, based on the results of comprehensive toxin concentration inhibition, proliferation and screening of tobacco callus. The screened disease-resistant mutant cells, the cell level of resistance and regeneration of plant disease resistance identification is consistent.