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目的探讨血管紧张素转换酶(ACE)基因多态性与2型糖尿病患者合并视网膜病变的关系。方法采用聚合酶链反应技术检测146例2型糖尿病患者ACE基因内含子16插入/缺失多态性,其中合并视网膜病变组27例,未发生视网膜病变组119例;同时选择200例健康志愿者作为正常对照组。结果合并视网膜病变组的ACE基因DD纯合型频率比未患视网膜病变组显著升高(70%与46%,P=0.042),但两组D等位基因频率比较无显著差异(80%与69%,P>0.05)。多因素分析表明,ACE基因DD基因型多态性与收缩压均为2型糖尿病视网膜病变的独立危险因素。结论ACE基因的DD基因型可增加2型糖尿病患者并发视网膜病变的危险性。
Objective To investigate the relationship between angiotensin converting enzyme (ACE) gene polymorphism and type 2 diabetic patients with retinopathy. Methods Polymerase chain reaction (PCR) was used to detect the insertion / deletion polymorphism of ACE gene in 146 patients with type 2 diabetes mellitus, including 27 cases with retinopathy and 119 cases without retinopathy; 200 healthy volunteers As a normal control group. Results The frequencies of DD homozygous ACE gene in patients with retinopathy were significantly higher than those without retinopathy (70% vs 46%, P = 0.042), but there was no significant difference in the frequency of D allele between the two groups (80% vs 69%, P> 0.05). Multivariate analysis showed that ACE genotype DD genotype polymorphism and systolic blood pressure were independent risk factors for type 2 diabetic retinopathy. Conclusion The DD genotype of ACE gene may increase the risk of retinopathy in type 2 diabetic patients.