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目的观察骨髓基质干细胞(B M SC s)在体外能否分化为软骨细胞。方法利用高密度细胞球培养体系在含转化生长因子-β1(TG F-β1)的培养基中培养B M SC s21d,用免疫组化甲苯胺蓝染色方法分析培养的B M SC s球中蛋白多糖(软骨细胞分泌的主要基质成份)的表达、用免疫组化和R T-P C R方法分析Ⅱ型胶原(软骨细胞特异分泌的主要胶原蛋白)的表达来评估B M SC s是否分化为软骨细胞。结果TG F-β1作用的B M SC s表达了Ⅱ型胶原和蛋白多糖。结论B M SC s在体外特定的培养条件下可分化为软骨细胞,从而可能成为临床上治疗创伤或骨关节炎所致的软骨缺损所需的合适的自体来源的种子细胞。
Objective To observe whether bone marrow stromal stem cells (BMSCs) can differentiate into chondrocytes in vitro. Methods BM SCs21d was cultured in culture medium containing transforming growth factor-β1 (TG F-β1) using high-density cell culture system. Proteoglycan in BM SCs was analyzed by immunohistochemical toluidine blue staining Chondrocytes), and the expression of type II collagen (the major chondrocyte-specific major collagen) was analyzed by immunohistochemistry and RTPCR methods to evaluate whether BMs differentiated into chondrocytes. Results The TG F-β1 treated B M SC s expressed type Ⅱ collagen and proteoglycan. Conclusion BMSCs can differentiate into chondrocytes in vitro under specific culture conditions and thus may be a suitable self-seeding seed cells for clinical treatment of cartilage defects caused by trauma or osteoarthritis.