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目的 :了解内蒙古地区蒙、汉族人 HLA-DRB1的基因多态性。方法 :采用聚合酶链反应 -序列特异性引物 (PCR-SSP)技术对内蒙古地区 1 0 2例健康蒙古族标本和 1 0 8例健康汉族标本进行 HLA-DRB1等位基因型别分析。结果 :蒙古族中等位基因频率较高的分别是 :DRB1 * 1 2 0 x(genefrequency,GF=1 8.3 5 % ) ,* 0 40 x(GF=1 4.82 % ) ,* 1 5 0 x(GF=1 0 .84% )。汉族中 HLA-DRB1等位基因频率较高的分别是 :DRB1 * 0 90 1 (GF=1 6.67% ) ,* 1 5 0 x (GF=1 5 .0 3 % ) ,* 0 40 x (GF=1 1 .81 % )。其中 :DRB1 * 0 90 1、* 1 2 0 x位点的基因频率差异有显著性意义。结论 :内蒙古地区蒙古族和汉族具有不同的 HLA-DRB1等位基因分布频率。
Objective: To understand the genetic polymorphism of HLA-DRB1 in Mongolian and Han nationality in Inner Mongolia. Methods: HLA-DRB1 allele was genotyped by polymerase chain reaction-sequence-specific primers (PCR-SSP) in 102 healthy Mongolian and 108 healthy Han nationality in Inner Mongolia. Results: The higher frequencies of Mongolian middle alleles were: DRB1 * 120 (genefrequency, GF = 1 8.3 5%), * 0 40 x (GF = 1 4.82%), * 150x = 1 0 .84%). The frequencies of HLA-DRB1 alleles in Han nationality were: DRB1 * 0 901 (GF = 1 6.67%), * 150x (GF = 15.30%), * 0 40x = 1 1 .81%). Among them, there were significant differences in the frequencies of genes between DRB1 * 0 901 and * 1 2 0 x loci. Conclusion: Mongolian and Han nationalities in Inner Mongolia have different distribution frequencies of HLA-DRB1 alleles.