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目的:从基因水平探讨抑癌基因p53 蛋白与鼻咽癌的关系。方法:本文采用Western blot 结合非放射性免疫沉淀技术,对鼻咽癌细胞株、鼻咽癌活检组织和鼻咽粘膜慢性炎症组织进行了p53 蛋白表达的研究。结果:7 株鼻咽癌细胞株均发现p53 蛋白表达异常条带。104 例鼻咽癌活检组织中有12 例出现p53 蛋白表达异常, 检出率为11.54 % 。8 例鼻咽慢性炎症活检组织中p53 蛋白表达未见异常。12 例p53 蛋白表达异常的鼻咽癌患者有2 例复发。结论:鼻咽癌组织中存在p53 蛋白表达异常,鼻咽癌的发生、发展可能与抑癌基因p53 蛋白的异常表达有关。
OBJECTIVE: To investigate the relationship between the tumor suppressor gene p53 protein and nasopharyngeal carcinoma at the gene level. Methods: Western blot and non-radioactive immunoprecipitation were used to study p53 protein expression in nasopharyngeal carcinoma cell lines, nasopharyngeal carcinoma biopsies and chronic inflammation tissues of nasopharyngeal mucosa. Results: Abnormal bands of p53 protein were found in all the seven nasopharyngeal carcinoma cell lines. In 104 cases of nasopharyngeal carcinoma biopsy tissue, 12 cases showed abnormal p53 protein expression, the detection rate was 11.54%. There was no abnormal expression of p53 protein in 8 cases of nasopharyngeal chronic inflammation biopsy. Twelve cases of nasopharyngeal carcinoma with aberrant expression of p53 protein had recurrence in 2 cases. Conclusion: There is abnormal expression of p53 protein in nasopharyngeal carcinoma. The occurrence and development of nasopharyngeal carcinoma may be related to the abnormal expression of p53 protein.