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目的观察双侧杏仁核同时点燃时疒间性发作的特点及出现时间,并探讨其机制。方法将40只成年雄性Wistar大鼠随机平均分为两组:双侧杏仁核同时点燃组(BK组)和单侧杏仁核点燃组(UK组)。在28d时间内,按照Goddard方法将大鼠点燃,并对结果进行对比分析。结果BK组所有大鼠在平均20.9次刺激后均出现Ⅴ级惊厥发作,点燃成功率100%,其中12只大鼠显示有自发性疒间性放电;UK组大鼠12只完全点燃,点燃成功率为60%,平均刺激次数为8.9次。两组相比双侧杏仁核点燃法可显著提高大鼠点燃成功率(P<0.01),但点燃时间明显延迟(P<0.01)。结论双侧杏仁核同时点燃可显著提高点燃成功率,并明显延缓点燃成功时间,可能与双侧点燃时神经元兴奋和抑制机制都显著增强有关。BK对点燃的致疒间机制和抗疒间药物的筛选研究提供了一种更为先进的动物模型。
Objective To observe the characteristics and the appearance time of bilateral amygdala simultaneously ignited, and to explore its mechanism. Methods Forty adult male Wistar rats were randomly divided into two groups: BK group and unilateral AL group (UK group). Within 28 days, rats were ignited according to Goddard’s method and the results were compared. Results All rats in group BK had grade Ⅴ episodes of seizures after an average of 20.9 stimuli, with a success rate of 100%. Twelve of the rats in group B showed spontaneous interscapular discharges. Twelve of the rats in UK group were completely ignited and were successfully ignited Rate was 60%, the average number of stimuli was 8.9 times. Compared with the bilateral amygdala lighting method, the two groups could significantly improve the success rate of ignition (P <0.01), but the ignition time was significantly delayed (P <0.01). Conclusions Simultaneous ignition of bilateral amygdala can significantly improve the success rate of ignition and significantly delay the ignition success time, which may be related to the significant enhancement of excitatory and inhibitory mechanisms of neurons during bilateral ignition. BK provides a more advanced animal model for the investigation of the mechanism of ignition and the screening of anti-tuberculosis drugs.