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目的 :探讨脑缺血再灌注损伤后脑内脑源性神经营养因子 ( BDNF) m RNA水平的变化规律 ,推测BDNF对损伤病理的影响。方法 :线栓法制作大鼠脑缺血再灌注模型 ,原位杂交检测大鼠海马神经元内BDNFm RNA,图象分析间接定量 BDNFm RNA水平。结果 :( 1 )脑缺血及缺血再灌注均能诱导双侧海马神经元BDNFm RNA水平增高 ;( 2 )缺血损伤过重后海马神经元 BDNFm RNA水平增高的程度反而小 ;( 3 )再灌注后BDNFm RNA的水平继续升高 ,高峰值出现在 1 2小时 ,至 72小时降至对照组水平 ;两侧海马的变化趋势类似。结论 :海马神经元在脑缺血及再灌注损伤后将升高 BDNFm RNA的表达以保护神经元 ,海马各区 BDNFm RNA基础水平与海马神经元病理改变程度呈负相关。
Aims: To investigate the changes of brain-derived neurotrophic factor (BDNF) m RNA in cerebral ischemia-reperfusion injury and to estimate the effect of BDNF on the injury pathology. Methods: The rat model of cerebral ischemia-reperfusion was established by thread occlusion method. BDNFm RNA was detected by in situ hybridization in rat hippocampal neurons. The BDNFm RNA level was indirectly quantified by image analysis. Results: (1) Cerebral ischemia and ischemia / reperfusion could both induce BDNFm RNA level increase in bilateral hippocampal neurons; (2) The level of BDNFm RNA in hippocampal neurons increased slightly after ischemic injury; (3) After reperfusion BDNFm RNA levels continue to rise, the peak appeared in 12 hours, 72 hours to the control group level; both sides of the hippocampus similar trends. CONCLUSION: Hippocampal neurons raise BDNFmRNA expression to protect neurons after cerebral ischemia and reperfusion injury. The basal levels of BDNFmRNA in hippocampus are negatively correlated with the pathological changes of hippocampal neurons.