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建立了同时测定牛奶中19种β-内酰胺类药物及其代谢物的高通量液相色谱串联质谱(UPLC-MS/MS)确证方法。以乙腈为提取溶剂,正己烷除脂后,采用C18填料分散固相萃取净化,在电喷雾正离子多反应监测模式下监测,19种化合物在6min内实现分离。方法的检测限为0.2～1μg/kg;方法的定量限为1～5μg/kg;在3个添加浓度水平时平均回收率为66%～107%,相对标准偏差为5.6%～15%。该方法可用于测定牛奶中19种β-内酰胺类药物及其代谢物。
A high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS / MS) method for simultaneous determination of 19 β-lactams and their metabolites in milk was established. Acetonitrile was used as the extraction solvent. After the n-hexane was degreased, C18 filler was used to disperse solid-phase extraction and purified. Electron spray positive ion monitoring was used to monitor the activity of 19 compounds. Sixteen compounds were separated within 6 minutes. The detection limit was 0.2 ~ 1μg / kg. The limit of quantification was 1 ~ 5μg / kg. The average recoveries were 66% -107% at 3 concentrations and the relative standard deviations were 5.6% ~ 15%. This method can be used to determine 19 kinds of β-lactam drugs and their metabolites in milk.