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采用超声技术从田基黄中提取活性物质。运用响应面法确定超声提取田基黄的最佳条件。运用单因素试验选取三个自变量。采用Box-Behnken设计评价这三个自变量对总黄酮和总多酚提取、DPPH和ABTS+活性清除的影响。通过方差分析显示,二次模型对反应的贡献率具有统计学意义。运用响应面法进行最优化研究,并用数学模型绘制出三维响应面。通过结合反应得出最佳试验条件:超声时间48.89 min,乙醇浓度63.72%,超声温度66.92℃。总黄酮值为105.06 mg RE/g DW,总多酚值为51.75 mg GAE/g DW,%DPPHsc为58.81%,%ABTSsc为64.99%。在最佳试验条件下,试验值与方差分析预测值一致。结果表明,采用四个模型和响应面法优化总黄酮和总多酚的提取、DPPH和ABTS+活性清除是切实可行的。
Ultrasound was used to extract the active substance from Tianji Yellow. Response Surface Methodology to Determine the Optimal Conditions for Ultrasonic Extraction of Tianji Yellow. Using the single factor test to select three independent variables. The Box-Behnken design was used to evaluate the effects of these three independent variables on total flavonoids, total polyphenols, DPPH and ABTS + clearance. Analysis of variance showed that the contribution of quadratic model to the reaction was statistically significant. Using response surface method to optimize research, and using mathematical model to draw the three-dimensional response surface. The optimal reaction conditions were as follows: ultrasonic time 48.89 min, ethanol concentration 63.72%, ultrasonic temperature 66.92 ℃. Total flavonoids were 105.06 mg RE / g DW, total polyphenol was 51.75 mg GAE / g DW,% DPPHsc was 58.81% and% ABTSsc was 64.99%. Under the best experimental conditions, the experimental values were in good agreement with the variance analysis. The results showed that using four models and response surface method to optimize the extraction of total flavonoids and total polyphenols, DPPH and ABTS + activity removal is feasible.