论文部分内容阅读
目的探讨IGF-IR反义寡核苷酸对食管癌EC9706细胞增殖、分化的影响。方法将4组终浓度为20μmol/L的3条封闭IGF-IR不同基因位点的反义寡核苷酸(ASODN1~3)及1条无关寡核酸(N-ODN)分组转染培养的食管癌EC9706细胞,另一组不转染;应用酸解DNA及甲绿-派洛宁染色技术观察各组食管癌EC9706细胞的增殖、分化情况。结果ASODN1~3转染组增殖型细胞分别为:56%、52%和61%,分化型细胞分别为:44%、48%和39%;N-ODN转染组及无转染组增殖型细胞分别为:82%和86%,分化型细胞分别为:18%和14%。对EC9706细胞的增殖、分化的影响,ASODN1~3组间相比差异无统计学意义(P>0.05),但ASODN1~3转染组与N-ODN转染组及无转染组相比,差异均有统计学意义(P<0.05)。结论IGF-IRASODN具有抑制食管癌EC9706细胞增殖,促进其分化效应。
Objective To investigate the effect of IGF-IR antisense oligonucleotide on the proliferation and differentiation of esophageal carcinoma EC9706 cells. Methods Four groups of antisense oligonucleotides (ASODN1 ~ 3) and one unrelated oligo-nucleic acid (N-ODN) were injected into three cultures of esophagus at final concentration of 20μmol / L The EC9706 cells were treated with EC9706 and the other groups were not transfected. The proliferation and differentiation of EC9706 cells in each group were observed by using acidolytic DNA and methylprednisolone staining. Results The number of proliferating cells in ASODN1 ~ 3 transfection group was 56%, 52% and 61%, respectively. The differentiated cells were 44%, 48% and 39% The cells were 82% and 86% respectively, and the differentiated cells were 18% and 14% respectively. There was no significant difference in the proliferation and differentiation of EC9706 cells between ASODN1 ~ 3 groups (P> 0.05). However, compared with the NODN1 ~ 3 transfected group and the non-transfected group, The differences were statistically significant (P <0.05). Conclusion IGF-IRASODN can inhibit the proliferation and promote the differentiation of EC9706 cells.