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为了解羊肚菌(Morchella esculenta)菌丝抗原物质的识别特征及其在菌丝上的分布,利用免疫酶染色法(Immunoenzyme assay,IEA)对两株抗羊肚菌单克隆抗体(W8C9,C6A8)在羊肚菌菌丝上对应的特异性结合靶点进行定位研究。初步确定了两株单克隆抗体对应的羊肚菌菌丝抗原在菌丝上的位置,并确定了W8C9、C6A8和兔多克隆抗体稀释后适合工作的体积分数分别为5.0%、4.5%、4.0%。
In order to understand the recognition characteristics of Morchella esculenta mycelium antigen and their distribution on mycelium, two monoclonal antibodies to Morchella esculenta (W8C9, C6A8 ) In the mycelium on the corresponding specific binding target for localization studies. The location of mycorrhizal fungi antigens on mycelium of two monoclonal antibodies was preliminarily determined, and the suitable volume fraction of W8C9, C6A8 and rabbit polyclonal antibodies after dilution was determined to be 5.0%, 4.5%, 4.0 %.