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背景:决明子具有降血压、降血脂、保肝及抑菌等活性,其减肥效应值得进一步探讨。目的:观察大鼠生理状态下自然饮用决明子水煎剂对营养性肥胖大鼠体质量的影响。设计:完全随机分组设计,对照实验,多组间比较采用方差分析和q检验。单位:河南科技大学第二附属医院心血管病研究室。材料:实验于2004-03/2004-09在河南科技大学第二附属医院心血管病研究室完成。选用雄性SD大鼠27只。将大鼠随机分为3组:正常对照组、模型对照组和决明子组,每组9只。方法:①正常对照组喂基础饲料(蛋白质、脂肪、碳水化合物含量分别是18.2%,4.5%,55.2%,每克含14.54kJ热量),自然饮水;模型对照组喂高营养性饲料(蛋白质、脂肪、碳水化合物含量分别是23.7%,21.6%,39.0%,每克含19.56kJ热量),自然饮水;决明子组喂高营养性饲料,自然饮不同浓度的决明子水煎剂。决明子水煎剂的浓度从10g/L(相当于每毫升含决明子生药10mg)开始,每天增加一个百分比浓度(即10g/L)直到第6天增至60g/L,从第7天开始保持60g/L的浓度不变直至实验第7周结束。②每天定时记录进食量和饮水量,计算摄入热量(进食质量×每克所含热量),每周定时测体质量。第7周末,测量大鼠体长,计算Lee’s指数3√体质量(g)×103/体长(cm)犦。③多组间比较采用方差分析和q检验。主要观察指标:决明子对营养性肥胖大鼠体质量、Lee,s指数、进食量、摄入热量和饮水量的影响。结果:大鼠27只均进入结果分析。①体质量:实验第3～7周模型对照组的明显高于正常对照组(P<0.05～0.01)。实验第2～7周决明子组明显低于模型对照组(P<0.05～0.01)。②Lee’s指数:实验第7周模型对照组和决明子组大鼠明显大于正常对照组犤(358.60±8.55),(341.84±7.29),(322.00±6.89)g/cm,P<0.05～0.01犦,决明子组明显低于模型对照组(P<0.05)。③进食量:模型对照组和决明子组明显低于正常对照组(P<0.05～0.01);决明子组与模型对照组相近(P>0.05)。④摄入热量:模型对照组和决明子组明显高于正常对照组(P<0.05～0.01)。决明子组与模型对照组相近(P>0.05)。⑤饮水量:决明子组基本与和模型对照组正常对照组相近(P>0.05)。模型对照组与正常对照组相近(P>0.05)。这表明60g/L质量浓度的决明子水煎剂不影响食欲。结论:质量浓度60g/L的决明子水煎剂既能明显抑制营养性肥胖大鼠体质量的增加,且不影响食欲。
BACKGROUND: Cassia has the functions of lowering blood pressure, lowering blood lipids, protecting liver and inhibiting bacteria, and its weight loss effect is worth further investigation. OBJECTIVE: To observe the effect of natural drinking cassia seed decoction on the body weight of rats with nutritional obesity under physiological conditions. Design: Completely randomized grouping design, control experiment, comparison between groups using analysis of variance and q test. Unit: Department of Cardiovascular Disease, Second Affiliated Hospital, Henan University of Science and Technology. MATERIALS: The experiment was performed at the Department of Cardiovascular Diseases, the Second Affiliated Hospital of Henan University of Science and Technology from March 2004 to September 2004. Twenty-seven male SD rats were used. Rats were randomly divided into 3 groups: normal control group, model control group and cassia seed group, with 9 rats in each group. Methods: 1 Normal control group fed basal diet (protein, fat, carbohydrate content were 18.2%, 4.5%, 55.2%, containing 14.54kJ calories per gram), natural drinking water; model control group fed high nutrient feed (protein, The fat and carbohydrate contents were 23.7%, 21.6%, and 39.0%, respectively, and contained 19.56kJ of heat per gram. Natural drinking water. Cassia seed fed highly nutritious feed and naturally consumed different concentrations of cassia decoction. The concentration of cassia seed decoction starts from 10g/L (corresponds to 10mg of crude drug containing cassia seed per ml), increases a percentage concentration (ie 10g/L) every day until 60g/L on the 6th day, and maintains 60g from the 7th day. The concentration of /L remained unchanged until the end of the experiment in the 7th week. 2 Regularly record the amount of food and drinking water every day, calculate the intake calories (intake quality × calories per gram), and measure body mass periodically every week. At the end of the 7th week, the body length of the rats was measured, and the Lee’s index 3 body weight (g)×103/body length (cm) was calculated. Three groups were compared using analysis of variance and q test. MAIN OUTCOME MEASURES: Effects of cassia seed on body weight, Lee, s index, food intake, caloric intake, and drinking water intake in nutritional obese rats. Results: All 27 rats were involved in the result analysis. 1 Body weight: The model control group was significantly higher than the normal control group at the third to seventh week of the experiment (P<0.05 to 0.01). The experimental group 2 to 7 weeks cassia seed group was significantly lower than the model control group (P <0.05 to 0.01). 2Lee’s index: At the 7th week of experiment, the rats in the model control group and cassia seed group were significantly higher than those in the normal control group (358.60±8.55), (341.84±7.29), (322.00±6.89) g/cm, P<0.05 to 0.01犦, cassia. The group was significantly lower than the model control group (P<0.05). 3 Food intake: Model control group and cassia seed group were significantly lower than normal control group (P<0.05-0.01); Cassia chinensis group was similar to model control group (P>0.05). 4 intake of calories: model control group and cassia seed group was significantly higher than the normal control group (P <0.05 ~ 0.01). Cassia seed group was similar to model control group (P>0.05). 5 Water intake: The cassia seed group was similar to the normal control group and the model control group (P>0.05). The model control group was similar to the normal control group (P>0.05). This shows that 60 g/L cassia decoction does not affect appetite. Conclusion: The cassia seed decoction with a mass concentration of 60 g/L can significantly inhibit the increase of body weight in nutritional obese rats without affecting appetite.