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本研究的目的是观察在猴前磨牙颊侧造成牙周组织缺损后,局部应用釉基质或其不同蛋白质成分对牙周组织再生的影响。 材料和方法 刮取6月龄猪仔双尖牙和磨牙胚的釉基质,制成匀浆,分别用0.5 tool/L冰醋酸和10％EDTA-Tris液提取成釉蛋白和釉蛋白,冻干。由于丙二醇藻酸盐(PGA)、羟乙基纤维素(HEC)和葡聚糖生物相容性好,能溶解釉基质蛋白,选作载体,与釉基质匀浆,成釉蛋白及釉蛋白等3种成分分别组合,制成不同制剂备用。 选择恒牙完全萌出、根尖孔封闭的成年猴,从双侧
The purpose of this study was to investigate the effect of local application of the enamel matrix or its different protein components on periodontal tissue regeneration following periodontal tissue loss caused by the buccal side of the premolar molars. MATERIALS AND METHODS The enamel matrix of 6-month-old piglet canines and molar teeth was scraped and homogenized. The ameloblastin and ameloblastin were extracted with 0.5mol / L glacial acetic acid and 10% EDTA-Tris solution, respectively. . As propylene glycol alginate (PGA), hydroxyethyl cellulose (HEC) and dextran good biocompatibility, can dissolve the enamel matrix protein, selected as a carrier, and the enamel matrix homogenate, ameloblasts and amelogenin 3 kinds of ingredients were combined, made of different preparations for use. Select permanent teeth fully erupted, apical foramen enclosed adult monkey from both sides