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为探讨氮素同化机理 ,小麦幼苗经溶液培养 (培养液中不含Ca2 + )至二叶一心后 ,在培养液中分别设置不同浓度的Ca2 + 、Ca2 + 专一性螯合剂EGTA以及Ca2 + 通道阻塞剂LaCl3 进行处理 (进行EGTA和LaCl3 处理时 ,培养液中Ca2 + 浓度为 1mmol/L)。结果表明 ,氮同化关键酶硝酸还原酶 (NR)、谷氨酰胺合成酶 (GS)活性同Ca2 + /CaM依赖性蛋白激酶 (Ca2 + /CaM PK)活性之间存在明显的相关性。说明钙作为第二信使所介导的信号转导中 ,蛋白质可逆磷酸化可能参与了对氮素同化的调节作用。
In order to explore the mechanism of nitrogen assimilation, wheat seedlings were cultured in solution (without Ca2 + in the culture medium) until the second leaf one heart, respectively, set in the culture medium different concentrations of Ca2 +, Ca2 + specific chelator EGTA and Ca2 + Channel blocking agent LaCl3 treatment (EGTA and LaCl3 treatment, the culture medium Ca2 concentration of 1mmol / L). The results showed that there was a significant correlation between NR and GS activity and Ca2 + / CaM-dependent protein kinase (Ca2 + / CaM PK) activity. The results showed that calcium as a second messenger mediated signal transduction, protein reversible phosphorylation may be involved in the regulation of nitrogen assimilation.