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目的探讨芜菁挥发油成分对高脂高糖小鼠动物模型的降血糖作用机制。方法采用超临界二氧化碳萃取仪提取芜菁挥发油成分。高糖高脂饲料喂养小鼠4周后,连续3 d腹腔注射链脲佐菌素(STZ)100 mg/kg,制备小鼠2型糖尿病模型,血糖测定仪尾静脉采血,测空腹血糖,小鼠血糖大于11.0 mmol/L即为造模成功。将小鼠随机分为空白对照组、模型组、芜菁挥发油高剂量[40 g/(kg·d)]组、芜菁挥发油低剂量[20 g/(kg·d)]组、阳性药物(盐酸二甲双胍)组,每日灌胃1次,连续14 d,分别于造模前,造模后,用药后3、7及14 d剪尾取血,检测血糖。小鼠摘眼球取血,血清测定糖化血红蛋白(Hb A1c)、甘油三酯(TG)、总胆固醇(TC)、血清胰岛素(INS)水平;剖取胸腺、脾脏,计算脏器指数。结果高、低剂量的芜菁挥发油均可降低2型糖尿病模型小鼠的血糖水平(P<0.01)及Hb A1c水平(P<0.05),升高INS水平(P<0.05),显著降低TG及TC水平(P<0.01),保护胸腺,增强机体的免疫能力。结论芜菁挥发油成分对链脲佐菌素联合高脂高糖小鼠糖尿病模型有较明显的降低血糖及血脂的作用,其机制可能是通过干预Hb A1c的形成、提高INS水平来降低高脂高糖小鼠血糖。
Objective To investigate the hypoglycemic mechanisms of volatile oil components in turnip on animal model of high fat and high glucose. Methods Supercritical carbon dioxide extraction was used to extract volatile oil from turnip. After 4 weeks of high-sucrose and high-fat diet, mice were given intraperitoneal injection of streptozotocin (STZ) 100 mg / kg for 3 weeks. The mice model of diabetes mellitus were prepared. Blood was collected from the tail vein of blood glucose meter to measure fasting blood glucose Rat blood glucose greater than 11.0 mmol / L is a successful model. The mice were randomly divided into blank control group, model group, high dose of volatile oil of turnip [40 g / (kg · d)], low dose of volatile oil of turnip [20 g / (kg · d)], Metformin Hydrochloride group). The rats were given gavage once a day for 14 consecutive days. Blood samples were taken before and 3, 7 and 14 days after model making, respectively. Blood samples were taken from the eyes of the mice and serum levels of Hb A1c, TG, TC and INS were measured. The thymus and spleen were taken and the organ index was calculated. Results High and low doses of volatile oil of turnip could decrease blood glucose level (P <0.01) and Hb A1c level (P <0.05), INS level (P <0.05) TC level (P <0.01), protect the thymus, enhance the body’s immune capacity. Conclusion The components of volatile oil of turnip have obvious effects on reducing blood glucose and blood lipid in diabetes model induced by streptozotocin combined with high fat and high glucose. The mechanism may be through the intervention of Hb A1c formation, increase of INS level and decrease of high fat and fat Sugar mouse blood sugar.