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为探明香蕉枯萎病菌侵染香蕉根系的过程,利用绿色荧光蛋白标记的香蕉枯萎病菌4号生理小种(Fusarium oxyspo-rum f.sp. cubense race 4 tagged with green fluorescent protein,GFP-FOC4),接种香蕉根系以观察病原菌侵染香蕉根系的组织学过程。结果表明,接种1 d后病原菌以菌丝体、大型分生孢子和小型分生孢子的形式附着于根系表皮细胞,优先沿细胞胞间层生长。接种7 d后,观察到病原菌以菌丝体、大型分生孢子和小型分生孢子的形式直接侵染维管束,在维管束内以两种方式扩展繁殖,一种在维管束内横向扩展,菌丝体随机分支,逐步形成网状分布;另一种是菌丝体在维管束内纵向生长,倾向于呈束状沿维管束单侧生长繁殖,形成大量菌丝体。本研究首次从组织病理学的角度观察并分析了GFP-FOC4侵染香蕉根系的过程,为研究香蕉枯萎病菌的致病过程机理提供参考。
In order to investigate the process of Fusarium wilt infection of Banana banana, Fusarium oxyspo-rum f.sp. cubense race 4 tagged with green fluorescent protein (GFP-FOC4) labeled with green fluorescent protein The banana roots were inoculated to observe the histological process of the pathogen infecting the banana roots. The results showed that on the 1st day after inoculation, the pathogenic bacteria adhered to the root epidermal cells in the form of mycelium, macro-conidia and small conidia, preferentially growing along the cell intercellular layer. Seven days after inoculation, pathogenic bacteria were observed to directly invade the vascular bundles in the form of mycelium, macroconidia, and small conidia, propagate within the vascular bundle in two ways, one that expands transversely within the vascular bundle, Mycelium randomly branched and gradually formed a network distribution; the other is the mycelium grow longitudinally in the vascular bundle, tend to bundle along the vascular bundle unilateral growth and reproduction, the formation of a large number of mycelium. In this study, we first observed and analyzed the process of GFP-FOC4 infection of banana root from the perspective of histopathology and provided a reference for studying the pathogenic mechanism of banana wilt pathogens.