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目的 :探讨FⅡG2 0 2 10A突变在中国人群中的分布频率及其与急性心肌梗塞之间的联系。方法 :运用FⅡG2 0 2 10A等位基因特异性引物PCR ,结合限制性内切酶技术及聚丙烯酰胺凝胶电泳等方法检测 97例急性心肌梗塞患者及 10 7例正常人对照的FⅡG2 0 2 10A基因频率。结果 :未检出 1例FⅡG2 0 2 10A基因突变者 ,提示被检病例及正常人FⅡG2 0 2 10A基因变异频率为 0。结论 :在中国人群中FⅡG2 0 2 10A基因变异罕见甚至缺如。FⅡG2 0 2 10A基因突变不足以成为中国人急性心肌梗塞的危险因素。
Objective: To investigate the distribution frequency of FⅡG2 0 2 10A mutation in Chinese population and its relationship with acute myocardial infarction. Methods: FⅡG2022A was detected in 97 patients with acute myocardial infarction and 107 normal controls by FⅡG202 10A allele-specific primer PCR, restriction endonuclease assay and polyacrylamide gel electrophoresis. Gene frequency. Results: One case of FⅡG2 0 2 10A gene mutation was not detected, suggesting that the mutation frequency of F ⅡG2 0 2 10A gene was 0 in both cases and normal subjects. CONCLUSION: The genetic variation of FⅡG20102A in Chinese population is rare or even absent. The mutation of FⅡG2012A is not enough to be a risk factor for acute myocardial infarction in Chinese.