论文部分内容阅读
目的应用DIGE联合飞行时间质谱测量法(MALDI-TOF)筛选和鉴定单绒毛膜发育受限胎儿(小胎)胎盘与发育正常胎儿(大胎)胎盘间差异蛋白,寻找潜在的单绒毛膜选择性胎儿生长受限的生物标记物。方法选择2015年7月至2016年6月于我院住院分娩的3例单绒毛膜选择性胎儿生长受限孕妇,对其胎盘组织中总蛋白采用DIGE联合飞行时间质谱测量法(MALDI-TOF)筛选和鉴定,得到表达差异蛋白。结果在生长受限的小胎胎盘组织中蛋白质表达上调的有13个,表达下调的有7个;大胎胎盘组织中钙调蛋白表达为0.22±0.10,小胎胎盘组织中钙调蛋白表达为0.82±0.33;大、小胎盘组织中PRDXⅢ表达分别为0.37±0.20与0.95±0.56,组间比较均具显著性差异(P<0.05)。结论 2D-DIGE技术能有效地筛选出单绒毛膜双胎s IUGR胎盘差异蛋白,为疾病的发病机制的研究提供了一种新的研究方法。
Objective To screen and identify differential proteins between the placenta of single chorionic development restricted fetus and normal fetal (large fetus) placenta using DIGE combined with time of flight mass spectrometry (MALDI-TOF) to find potential monocentromembrane selectivity Fetal growth restriction biomarkers. Methods From March 2015 to June 2016, 3 cases of single chorionic selective fetal growth restriction in our hospital were enrolled in this study. Total placental tissue protein was detected by DIGE combined with time of flight mass spectrometry (MALDI-TOF) Screening and identification, the expression of differentially expressed proteins. Results The expression of protein in up-regulated small placenta tissue was 13, and down-regulated in 7 placenta tissues. The expression of calmodulin was 0.22 ± 0.10 in the placenta of large placenta. The expression of calmodulin was 0.82 ± 0.33. The expression of PRDX Ⅲ in large and small placenta was 0.37 ± 0.20 and 0.95 ± 0.56, respectively, with significant difference between the two groups (P <0.05). Conclusion 2D-DIGE technique can effectively screen placental differentially expressed proteins of single chorionic twins, which provides a new method for the study of the pathogenesis of the disease.