论文部分内容阅读
利用高培养力基因型与甜玉米杂交，其Ｆ１代进行花药培养具有较高的培养力，花粉胚的诱导频率可达２６％．甜玉米基因型通过幼胚培养，建立起２个可长期继代培养，大量再生绿苗的体细胞克隆，并获得了自交结实后代，同工酶分析表明，同一克隆再生植株可产生基因突变．常规高培养力材料与甜玉米杂交后代进行二环系培养，可以筛选出高配合力的超甜玉米自交系。
The high-cultivating genotype was crossed with sweet corn. The F1 generation of anther culture had higher culture ability, and the frequency of pollen embryo induction was up to 26%. In the immature embryo culture, two sweet corn genotypes were used to establish somatic cell clones capable of long-term subculture and regeneration of green shoots in large quantities. Self-fertilizing and fruiting progenies were obtained. Isoenzyme analysis showed that the same clone regenerated plants could produce gene mutations . Conventional high culture materials and sweet corn hybrid progeny for the second ring culture, can be screened with the ability of super sweet corn inbred lines.