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目的:探讨中药人参和泼尼松对关木通所致急性肾毒性的干预作用。方法:54只清洁级雌性SD大鼠随机分组,以关木通煎剂40 g.kg-1.d-1制备急性肾毒性模型。分别给予泼尼松(30 m g.k-g 1.-d 1)和生晒参煎剂(40 g.k-g 1.-d 1)灌胃5 d;第4和8 d测定血、尿生化等指标,免疫组化染色进行肾组织病理学观察;用SPSS11.5软件进行统计学处理。结果:含关木通各组大鼠体重和活动能力下降,而血尿素氮(BUN)和肌酐(C r)、尿N乙酰葡糖胺(NAG)酶、尿2β微球蛋白(2βM G)、转化生长因子β1(TGFβ1)、Ⅳ型胶原均有不同程度增高,其中关木通组差异最大;与单纯关木通组比较,各实验组BUN、C r、尿NAG酶、TGFβ1、Ⅳ型胶原有不同程度的降低。结论:单独用人参或泼尼松与关木通混合灌胃,或同时用人参、泼尼松和关木通混合灌胃,均能减轻关木通对大鼠的急性肾毒性。
Objective: To investigate the intervention of traditional Chinese medicine ginseng and prednisone on acute nephrotoxicity induced by Guanmutong. Methods: Fifty-four female Sprague-Dawley rats were randomly divided into three groups: Acute nephrotoxicity model was established with 40 g · kg-1.d-1 of Guanmutong decoction. The rats were given gonadotrophin (30 m gk-g 1.-d 1) and raw sun-ginseng decoction (40 gk-g 1.-d 1) orally for 5 days respectively. Blood and urine biochemistry were measured on the 4th and 8th day Indicators, immunohistochemical staining of renal tissue pathological observation; SPSS11.5 software for statistical analysis. Results: The body weight and activity of rats in Guanmutong group decreased, while BUN and C r, NAG enzyme, urine 2β microglobulin (2βM G) TGFβ1 and typeⅣcollagen increased in varying degrees, among which the difference was greatest in Guanmutong group. Compared with simple Guanmutong group, BUN, C r, urinary NAGase, TGFβ1 and typeⅣcollagen in each experimental group decreased to some extent . Conclusion: Ginseng alone or prednisone combined with Guanmutou gavage, or both ginseng, prednisone and Guan Mu Tong gavage, can reduce the acute Guanyuan Tong rats acute nephrotoxicity.