论文部分内容阅读
目的探讨幼年和成年Wistar大鼠脑干胶质瘤侵袭性的差异。方法 50只幼年和成年Wistar大鼠随机分4组,A组(n=15)和C组(n=15)分别将大鼠C6胶质瘤细胞注射到幼鼠和成鼠脑桥,B组(n=10)和D组(n=10)大鼠分别注射等体积生理盐水到幼鼠和成鼠脑桥。2周后MRI观察肿瘤生长情况并测量体积,常规行苏木精-伊红染色观察肿瘤组织形态学变化,免疫组织化学染色检测细胞侵袭相关因子基质金属蛋白酶2(MMP-2)、基质金属蛋白酶9(MMP-9)和β-catenin的表达。记录4组大鼠生存时间。结果 MRI检查结果:A、C组在脑桥均发现肿瘤,肿瘤平均体积分别为(49.63±8.34)mm3和(52.07±7.77)mm3,两者无统计学差异(P>0.05);B、D组无肿瘤生长。A、C组大鼠平均生存时间分别为(19.47±2.23)d和(21.47±2.23)d,统计学比较有显著性差异(P<0.05);B、D组大鼠至另2组大鼠全部死亡时仍存活。A、C组肿瘤形态观察及MMP-2、MMP-9、β-catenin的表达无明显差异(均P>0.05)。结论成功建立幼年和成年大鼠脑干胶质瘤动物模型,但二者肿瘤侵袭性无明显差异。
Objective To investigate the difference of invasiveness of brain stem glioma in young and adult Wistar rats. Methods Fifty young and adult Wistar rats were randomly divided into 4 groups: group A (n = 15) and group C (n = 15), respectively. C6 glioma cells were injected into the pons of young rats and adult rats, n = 10) and D group (n = 10) rats were injected with equal volume of saline to the pups and rats pons. The tumor growth was observed 2 weeks later and the volume was measured. The histological changes were observed by hematoxylin-eosin staining and the expression of matrix metalloproteinase-2 (MMP-2), matrix metalloproteinase 9 (MMP-9) and β-catenin expression. Four groups of rats were recorded for their survival time. Results The results of MRI examination showed that tumors were found in the pons in groups A and C, respectively. The mean volume of tumors was (49.63 ± 8.34) mm3 and (52.07 ± 7.77) mm3 respectively, with no significant difference (P> 0.05) No tumor growth. The average survival time of rats in group A and group C was (19.47 ± 2.23) d and (21.47 ± 2.23) d, respectively, with statistical significance (P <0.05). Rats in groups B and D to the other two groups All died while still alive. The morphological changes of A, C group and the expression of MMP-2, MMP-9 and β-catenin had no significant difference (all P> 0.05). Conclusion The animal model of brainstem glioma was successfully established in both young and adult rats, but there was no significant difference in tumor invasiveness between the two groups.