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目的 探讨大鼠消化道是否存在 Gn RH受体 m RNA及其定位。 方法 用原位杂交组织化学法。 结果 胃底腺壁细胞、胃小凹上皮细胞可检测到较强的 Gn RH受体 m RNA杂交信号。3段小肠绒毛上皮细胞、小肠腺细胞可检测到较强的 Gn RH受体 m RNA杂交信号。盲肠、结肠和直肠的粘膜上皮细胞、肠腺上皮细胞也能检测到 Gn RH受体 m RNA杂交信号。信号物质均分布在胞质内 ,胞核呈阴性。 结论 大鼠消化道能合成 Gn RH受体。Gn RH也是一种胃肠激素 ,它由消化系统自身合成 ,又作用于胃肠道参与胃肠功能的调节
Objective To investigate the presence and location of Gn RH receptor m RNA in the digestive tract of rats. Methods In situ hybridization histochemical method. Results The Gn RH receptor m RNA hybridization signal was detected in gastric parietal gland cells and gastric pits epithelial cells. Three segments of small intestinal villi epithelial cells, small intestine gland cells can detect strong Gn RH receptor m RNA hybridization signal. Cecum, colon and rectal mucosal epithelial cells, glandular epithelial cells can also detect Gn RH receptor m RNA hybridization signal. Signal substances are distributed in the cytoplasm, the nucleus was negative. Conclusion Gn RH receptors can be synthesized in the digestive tract of rats. Gn RH is also a gastrointestinal hormone that is synthesized by the digestive system itself and acts on the gastrointestinal tract to participate in the regulation of gastrointestinal function