论文部分内容阅读
分别将元宫铜365,元宫铜300及元宫药铜220宫内节育器浸泡在37℃的50mL的模拟宫腔液中,定期换液.用原子吸收火焰法,及紫外可见分光光度计分别测试了模拟宫腔液中的铜离子浓度及吲哚美辛浓度,并用扫描电子显微镜观测了3种元宫宫内节育器在模拟宫腔液中浸泡1天及30天后的铜丝表面形貌.此外还用MTT法评价了这3种元宫铜宫内节育器对L929小鼠成纤维细胞的细胞毒性.从铜离子释放速率曲线,吲哚美辛释放速率曲线及浸泡1天及30天后的铜丝表面形貌可以得出铜离子及吲哚美辛的释放都是前期快,后期慢.吲哚美辛的释放可以加速铜离子的释放速率.元宫铜宫内节育器的铜丝表面呈现明显的不均匀腐蚀现象.元宫铜宫内节育器铜丝与不锈钢丝接触的部分由于与模拟宫腔液很少接触,故腐蚀程度小,而铜丝与模拟宫腔液接触的部分在通常状态下由于电偶腐蚀铜离子释放加速.这3种元宫铜宫内节育器的细胞毒性都为4级.应从实际实验中仔细研究Cu-IUD的副作用,尽力减小其毒性.
Respectively, Yuan Gong Tong 365, Yuan Gong Tong 300 and Yuan Gong Yao 220 IUD were soaked in 50 mL simulated uterine fluid at 37 ℃, regular liquid exchange. Using atomic absorption flame method, and UV-visible spectrophotometer The concentrations of copper ions and indometacin in uterine fluid were simulated and the surface morphology of copper wires of three kinds of uterine IUDs immersed in simulated uterine fluid for 1 day and 30 days were observed with scanning electron microscopy In addition, the MTT method was also used to evaluate the cytotoxicity of these three kinds of uterine copper IUDs on L929 mouse fibroblasts.From the curve of copper ion release rate, the release rate curve of indomethacin and soaking for 1 day and 30 Day after the copper surface morphology can be concluded that the release of copper ions and indomethacin are pre-fast, late slow release of indomethacin can accelerate the release rate of copper ions. Silk surface showed significant uneven corrosion.Yuan Palace copper IUD copper wire contact with the stainless steel wire due to little contact with the simulated uterine fluid, so the degree of corrosion is small, and the copper wire and simulated fluid contact with the uterine cavity Part of the copper ion release due to galvanic corrosion under normal conditions Speed. These three per house cytotoxic copper IUD are four. Actual experiment should examine Cu-IUD side effects, toxicity reduced to the utmost.